Global quantification of protein expression (‘Discovery’ type experiments)

By applying mass spectrometry based proteomics, we can analyse almost any biological sample, including cell lines, tissue, formalin fixed paraffin embedded tissue, bio-fluids, plants, fungi, and more. 

Our main application is quantification of proteins using label free or SILAC based methods, in simple and complex biological samples. ‘Discovery’ type experiments can yield as many as 9,000 proteins, depending on the type of sample. We use a variety of data acquisition modes such as DDA and DIA.

Analysis of protein-protein interaction

Analysis of samples after Co-IP/pulldown for identification of protein-protein interactions and/or posttranslational modifications. 

Global quantification of the phosphoproteome (Fe-IMAC)

Enrichment of phosphopeptides using Immobilised Metal Affinity Chromatography on an automated platform. From 100ug protein we can identify and quantify over 10,000 phosphopeptides.

Targeted quantification of proteins and PTMs (PRM)

A powerful mass spectrometry technique for specific and sensitive quantification of proteins and their PTMs. Targeted quantification is suitable for hypothesis driven experiments, where the proteins of interest are already known.


We use our novel glycoproteomics workflow, which includes glycopeptide enrichment, data acquisition and software tools for global analysis of glycoproteins. 

It is most applicable for human and mouse, since it relies on a glycan database. 

Intact protein analysis

Analysis of intact proteins for MW determination

Global detection of ubiquitination sites (using anti k-GG enrichment)

We use the CellSignaling antibody to enrich peptides carrying the GG remenant (after digestion with trypsin). 

Analysis of serum/plasma including immunodepletion

We deplete the abundant proteins and then analyze by mass spec based proteomics as usual. 

Analysis of histone modifications


Crosslinking mass spectrometry

Anaylsis of crosslinked proteins for identification of interaction sites. 

Global analysis of S-nitrosylation (SNO-RAC method)

Enrichment of S-nitrosylated peptides using the SNO-RAC method.