Global quantification of protein expression (‘Discovery’ type experiments)

We can analyse almost any biological sample, including cell lines, tissue, formalin fixed paraffin embedded tissue, bio-fluids, plants, fungi, and more. 

Our main application where we aim to quantify as many proteins as possible using either label free or SILAC based methods, in simple and complex biological samples. ‘Discovery’ type experiments can be performed using either 1DLC, where the typical coverage is ~3,000 proteins from ~3e5 cells. When using 2DLC(prefractionation), we can reach as many as 9,000 proteins from 1e6 cells.

Analysis of protein-protein interactions
Analysis of samples after Co-IP/pulldown.

Global quantification of the phosphoproteome (Fe-IMAC)

Enrichment of phosphopeptides using Immobilised Metal Affinity Chromatography on an automated platform. From 100ug protein we can identify and quantify over 10,000 phosphopeptides.

Targeted quantification of proteins and PTMs (SRM, PRM):

A powerful technique for specific and sensitive quantification of proteins and their PTMs. Targeted quantification is suitable for hypothesis driven experiments, where the proteins of interest are already known.

Intact protein analysis

Analysis of intact proteins for MW determination

Global detection of ubiquitination sites (using anti k-GG enrichment). 

Analysis of serum/plasma including immunodepletion
Immunodepletion of abundant proteins using the MARS14 column, followed by either targeted or discovery analysis.

Analysis of histone modifications.

Global analysis of S-nitrosylation (SNO-RAC method)
Enrichment of S-nitrosylated peptides using the SNO-RAC method.