The Crown Institute for Genomics Platform provides NGS (Next-Generation Sequencing) services for samples prepared by the Platform or for libraries submitted by external users.

Library preparation

The Crown Institute for Genomics supports a wide range of library preparation protocols and sequencing options.
In addition to standard Illumina kits, we developed and offer high quality in-house protocols for major applications at a reduced cost.
 

RNA-seq

  • Full length RNA-seq - polyA-based capturing
  • Full length RNA-seq - without polyA capture
  • 3' RNA-seq - Bulk MARS-seq
  • Turseq stranded RNA - Illumina
  • Small RNA (stranded, 17-22bp) - Illumina

 

DNA-Seq

  • Whole Genome Sequencing
  • PCR-Free

 

ChIP-seq

  • Fragmented DNA or amplicons < 500bp

 

    Single Cell Genomics

    • Single cell 3' RNA-seq - 10x genomics
    • Cell Hashing
    • Single cell 5' RNA-seq - 10x genomics
    • Single cell immune profiling - TCR/BCR sequencing

     

    Long read sequencing

    PacBio, Sequel - Coming soon

     

    Sequencing

    We sequence libraries that were prepared in our facility libraries or prepared by the researcher (Ready to Run).

    We offer a variety of sequencing instruments:

    • Novaseq 6000 – high throughput sequencing, >800M reads per run
    • NextSeq 500 – medium throughput sequencing, 400M reads (HO) or 130M reads (MO) per run
    • MiSeq – low throughput sequencing, 1M, 4M, 12M, 22M reads per run