The Crown Institute for Genomics Platform provides NGS (Next-Generation Sequencing) services for samples prepared by the Platform or for libraries submitted by external users.

Library preparation

The Crown Institute for Genomics supports a wide range of library preparation protocols and sequencing options.
In addition to standard Illumina kits, we developed and offer high quality in-house protocols for major applications at a reduced cost.


  • Full length RNA-seq - polyA-based capturing
  • Full length RNA-seq - without polyA capture
  • 3' RNA-seq - Bulk MARS-seq
  • Turseq stranded RNA - Illumina
  • Full length RNA-seq low input - Bulk smart-seq2



  • Whole Genome Sequencing



  • Fragmented DNA or amplicons < 500bp


    Single Cell Genomics

    • Single cell 3' RNA-seq - 10x genomics
    • Cell Hashing
    • Single cell 5' RNA-seq - 10x genomics
    • Single cell immune profiling - TCR/BCR sequencing
    • Single cell ATAC-seq
    • Single cell multiome analysis

    For more information visit


    Spatial transcriptomics

    • Visium fresh frozen - 10x genomics
    • Visium FFPE 

    For more information visit



      We sequence libraries that were prepared in our facility libraries or prepared by the researcher (Ready to Run).

      We offer a variety of sequencing instruments:

      • Novaseq X - high throughptu sequencing, 10B, 25B reads per run
      • Novaseq 6000 – high throughput sequencing, >800M reads per run
      • NextSeq 500 – medium throughput sequencing, 400M reads (HO) or 130M reads (MO) per run
      • MiSeq – low throughput sequencing, 1M, 4M, 12M, 22M reads per run


      Long read sequencing

      • PacBio, Sequel