We can use a 'bottom-up' proteomics approach where proteins are extracted from the biological samples, subjected to enzymatic digestion followed by liquid chromatography - mass spectrometric analysis. Post-acquisition, the protein identity and quantity is reconstructed using cutting edge bioinformatics. Or we can perfrom top down analysis of small proteins using our Fusion Lumos mass spectrometer, equipped with multiple fragmentation technologies. We apply rigorous quality control and criteria for generating the best data from our instruments.
Clip 1: discovery workflow: sample preparation and instrumentation (3:41min)
Clip 2: discovery workflow: data processing (4:12min)
Clip 3: understanding the result file
In a ‘targeted’ type experiment we quantify a specific set of proteins using their serrate tryptic peptides. This is conducted using synthetic, stable heavy isotopically labeled peptide standards. The analysis is conducted either on our tandem quadrupole mass spectrometer, in Selective Reaction Monitoring (SRM) mode or on our high resolution quadrupole Orbitrap mass spectrometer, in Parallel Reaction Monitoring mode (PRM). The main advantage of these techniques is the sensitive, specific and reproducible quantification of peptides and proteins.