- How long does the analysis take?
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Our instruments run samples 24/7 continuously including holidays and weekends. Occasionally they are down for maintenance or troubleshooting. Currently we can complete a typical experiment between 2 to 5 weeks, from submission of samples to receiving the data. Occasionally it takes longer in cases such as complex experiments or due to technical reasons.
- What is included in a typical analysis?
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We perform the sample preparation, LC-MS/MS analysis and data processing. Pathway analysis is not included.
- What proteomic coverage can you reach?
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From mammalian cells and tissue, we can identify and quantify between 2,000 to 9,000 proteins depending on the type of analysis and amount of extracted protein.
- What is the best Co-IP method for investigation of protein-protein interactions?
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Please refer to these guidelines. In our experience, the FLAG system using anti-FLAG antibodies produces the least non-specific binding and the best results. However we have worked with His, HA, GST, biotin-streptavidin tags as well as antibodies against the endogenous bait.
Please consult with us at the experimental design stage. - How much protein is required for analysis?
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This various depending on the application. For whole lysates, we can work with extracts from 3e5 cells or more.