G-INCPM-Special Seminar - Prof. Govert Somsen, Division of BioAnalytical Chemistry, Vrije University, Amsterdam - "Probing the heterogeneity of intact proteins"Date:
Lecture / Seminar
Location: Nancy and Stephen Grand Israel National Center for Personalized Medicine
Lecturer: Prof. Govert Somsen
Organizer: Department of Life Sciences Core Facilities
Details: University of Amsterdam
Abstract: Emerging requirements in biopharmaceutical quality control and top-down proteomi ...Emerging requirements in biopharmaceutical quality control and top-down proteomics, have led to a growing interest in intact protein analysis. Detailed characterization of intact protein heterogeneity, e.g. resulting from post-translational modifications, is a challenging task. Separation prior to mass spectrometric (MS) detection commonly is essential to achieve reliable and sensitive assignment of intact protein variants. Conventional liquid chromatographic (LC) protein separation methods often show poor compatibility with MS and/or lack the selectivity to resolve proteoforms. Moreover, LC conditions may be denaturing, precluding assessment of protein conformers and proteoform affinity. This lecture presents the design and application of new LC-MS and capillary electrophoresis (CE)-MS methods allowing detailed determination of the heterogeneity of intact proteins. Performance will be illustrated by characterization of pharmaceutical proteins, such as interferon-beta, erythropoietin, and monoclonal antibodies (mAbs), as well as cell-lysate proteins and IgGs in human plasma.