Crown Institute for Genomics


MiSeqThe MiSeq desktop sequencer enables a broad range of applications, such as targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression, amplicon sequencing, and HLA typing. MiSeq is the only desktop sequencer that can produce 2 x 300 paired-end reads in a single run.

MiSeq specifications:

MiSeq reagent kit Read length Output Reads passing filter (paired-end reads)



2x25 bp 750-850 Mb

30-34 M

2x150 bp 4.5-5.4 Gb
2x250 bp 7.5-8.5 Gb


2x75 bp 3.3-3.8 Gb 44-50 M
2x300 bp 13.2-15 Gb