Crown Institute for Genomics

cBot

cBotcBot is an automated system that creates clonal clusters from a single molecule DNA templates, preparing them for sequencing by synthesis on the HiSeq sequencer.cBot isothermally amplifies cDNA fragments that have been captured by complementary adapter oligonucleotides covalently bound to the surface of Illumina flow cells. Flow cells facilitate access of bound DNA to enzymes. Attached DNA fragments are extended and bridge amplified to create hundreds of millions of clusters, each of which contains ~1000 identical copies of a single template molecule.