Crown Institute for Genomics

Single cell RNA-seq at the G-INCPM

Single-cell RNA-seq provides a high resolution measurement that allows to differentiate and study individual cells within heterogeneous populations. This technology enables the classification and characterization of different cell types within a bulk.

Main features:

  • Full transcript library prep and sequencing using the Smart-Seq2 protocol1
  • Medium throughput experiments (dozens to thousands of cells).
  • Typically, 8k-10k transcripts per cell (in mammalian cells). 
  • Compatible with FACS sorting for targeted cell populations. 
  • Minimal cell loss: typically, >80% of sorted cells are successfully prepared and sequenced.
  • Separation between sorting and library preparation - FACS sorting into 384 well plates is performed at the user facility, and the frozen plates are delivered when convenient. 

In this workflow, single cells are sorted by the user into 384-well plates, provided in advance, containing a lysis buffer. Following sorting, the plates are frozen and delivered at the user’s convenience. Library preparation and sequencing are performed at the G-INCPM. We offer single-cell analysis services, including data processing, QC, sub-population discovery, marker identification, and differential expression.

Please fill in the project request form in order to arrange a consultation meeting.

1Picelli, S et al., Full-length RNA-seq from single cells using Smart-seq2. Nature Protocols, 2014